赛默飞世尔Thermo Fisher 100 LITER P

小RNA的Northern BLOT

cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick)  Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel:  Add 678 μl

SSR GEL and Silver Staining Protocol

to first direction, don't use too much pressure. This treatment needs to be repeated every time.   III. GEL SOLUTION PREPARATION: 1. Rinse all glassware and plastic ware with d.i. water prior to gel solution preparation and pouring, including

SSR GEL and Silver Staining Protocol

for 2 min., ice and 5 μl 1X PE II buffer 2.5 μl of 400 ng / μl of sonicated Poly-dA 7.5 μl SSRP bufferVI. ELECTROPHORESIS:Note: Adding sodium acetate to the bottom reservoir during electrophoresis (Sheen and Seed, 1988, Biotechniques 6:942-944) produces