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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
PREPARATION OF MICROINJECTION PIPETTES
Using Program 6 (VH) Heat = 915 Pull = 20 Vel = 90 Time = 120 Pressure = 500 Injection pipettes:- Injection pipettes should have an internal diameter only marginally larger than the stem cells (18-20 um) and a sharp point which allows
Preparation of Microinjection Pipettes
a few days. Pipette pulling: Injection pipettes: Injection pipettes should have an internal diameter only marginally larger than the stem cells (18-20 um) and a sharp point which allows the needle to penetrate cleanly in the blastocoel
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