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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of methods used to uncover insertion sites have previouslybeen described, including genomic DNA library screening(15), ligation-mediated PCR (6), inverse PCR (16), VISAtechnique (17), T-linker PCR (18), and single nucleotidepolymorphism-based mapping (19
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
might break otherwise. Centrifuge homogenate at 3,000g (5,000 r.p.m. in a Sorvall SS-34 rotor) for 10 min at 4 °C. Transfer the supernatant into a fresh tube and discard the pellet. Centrifuge the supernatant at 32,000g (16,500 r.p.m
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