赛默飞世尔Thermo Fisher FUNNEL PP V

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

Preparation of Tonsil Lysate

experiment.Disslve in 100% EtH) 1 mM Benzamidine 5 mM Idacetamide Whatman Paper Centrifuge Bttles Funnel Squeeze bttle Several Beakers Hmgenizer (Fisher PwerGen 125) 50ml Crning tubes Ice Bucket & Ice Dunce hmgenizer (40ml Pyrex Cat N.7727

A quick RNA mini-prep for Neurospora mycelial cultures

x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C. A 50 ml culture typically yields enough RNA