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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70311785254
中文名称:
英文名称:X8 Flask Millicell HY T-1000 cell culture, 5 layer, sterile, 10,000mm2 surface area 12mm x
货期:热销品现货,其他请咨询




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文献和实验and will remain in contact with the bottom of the culture flask. They will continue to divide and migrate until the entire bottom of the flask is covered with a single layer of cells (contact inhibition and the formation of a monolayer). Chapter 12: Cell Cultures
Establishment of a Primary Culture
in MEM at 1000 rpm for 10 minutes in a standard clinical centrifuge. Remove the supernatant and resuspend the pellet in 25 ml of fresh MEM + 10% FCS. Remove 0.1 ml of the culture and determine cell concentration and viability
Density sensitive - die if too dense or too dilute III. MAINTENANCE Split one flask of cells (most recent date) into two new T-75 flasks (VWR# BD353136) when culture is confluent. Keep other flask as a backup. Monitor growth status by microscopy
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