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文献和实验Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
or commercial NO (Fisher) Methyl red 0.1 M sodium phosphate buffer, pH 7.40 ( appendix 2A
AFLP: not only for fingerprinting, but for positional cloning
acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs
AFLP: not only for fingerprinting, but for positional cloning
within 40 Kb of each other, on average. This level of physical linkage would result is the generation of markers that are _ 0.3 cM (0.3% recombination) within the mutant locus of interest. Thus AFLP should eliminate the "walk" typically associated with non-T
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