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货号：T_70311708001
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英文名称：X6 VOL PIPETTE 10 ML CL AS
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文献和实验, a pair of frogs were mated (see Grainger Lab X. tropicalis Mating Protocol for details) and the resultant tadpoles were used in the following karyotyping protocol. 2. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
RUVKUN Antibody Staining Protocol revised 10/29/90
above (total volume= 5ml). Then add heptane(or water) and MeOH; EGTA. 100mM Tris Cl pH 7.4 1% Triton X-100 1 mM EDTA 1M H3BO3 0.5 M NaOH 0.1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA
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