赛默飞世尔Thermo Fisher 125 ML S/S

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells

Orbital Shaker • Water Bath, VWR Scientific • 500 ml Vented Cap Erlenmymer Flask, • 125 ml Vented Cap Erlenmymer Flask, Corning product • Pro293S-CDM, 1 L (Cambrex Biosciences, Cat 12-765Q) • Penicillin-Streptomycin, Invitrogen • Transfection

Cell Cultures

media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml