赛默飞世尔Thermo Fisher X10 BOTTLE

原创：western protocol

antibody: Anti-mouse IgG-Alkaline Phospphatase Buffers Lysis buffer: 100ml 50 mM Tris-Cl, pH 8.0 0.6057g 150m M NaCl 0.8766g 0.02%NaN3 0.02g 100ug/mlPMSF 0.01g 10ug/ml Aprotinin 0.001g 1% Triton X100 1g 2x SDS-loading buffer: 100ml 100 mM Tris

Loss of Heterozygosity

µl of 10% ammonium persulfate to 75 ml of Gel-mix-6. Mix by inversion. Hold the nozzle of the bottle at the corner of the gel cast. Hold the gel cast at a 45o angle to the bench and pour the gel between the plates. If bubbles get trapped

Preparation of Tonsil Lysate

Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh