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货号：T_70113-681-509
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英文名称：REAGENT RESERVOIR 25ML ST BULK
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文献和实验Cloning by Limiting Dilution of Hybridoma
according to the calculated dilutions. Serially dilute each clone to 4, 2, and 1 cell/ml. The final dilution tube should contain 50 ml of cloning medium at 1 cell/ml. Pour each of the dilutions into a sterile reagent reservoir. Plate 250 ml/well into 96-well plates
Cloning by Limiting Dilution of Hybridoma
, Inc. #25030-149 or equivalent) Hybridoma Cloning Factor (Fisher # IG50-0615) 50 ml sterile centrifuge tubes ( Falcon #2070) 15 ml sterile centrifuge tubes (Falcon
Fusion of Mouse, Rat, or Hamster Cell
#3046) Hemocytometer Trypan Blue, 0.4 (Life Technologies, Inc. # 630-5150AG) Multi-channel pipettor and sterile tips Reagent Reservoir HT (Life
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