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文献和实验et al., ) Ice Magnesium chloride (Fisher Scientific)
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
Chlamydomonas in a culture tube. Prepare a vaseline mount of the culture and observe the living organisms. You'll need to use dark field at a low power (100x) to locate them, as they are very small and very fast. Zoom in on one or more cells that aren't moving
【转贴】immunofluorescence staining
). Remove and save Secondary Antibody.Wash X 5 with TBS-T, then wash with PEM.NOTE: Sodium azide is toxic and can cause corrosion of plumbing-flush sinks with large volumes of water. Do not use Sodium azide if the secondary antibody will be HRP labeled
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