赛默飞世尔Thermo Fisher X1152 1100µ

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡

in 4% paraformaldehyde to give a working concentration of 10 µg/mL.  Discard whatever is not used on the day of use.  6)      Grid plates � Purchase from Fisher Scientific (#08-757-149) 7)      Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain

Southern Hybridization Protocols

Primers Solution 7 µ l distilled water Mix briefly. Pipette up and down a tip of alpha - 32P with the above mixed solution until the reddish color on pipette tip disappears. Add 1 µ l Klenow fragment, centrifuge briefly