赛默飞世尔Thermo Fisher X960 0,5-10

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡

in 4% paraformaldehyde to give a working concentration of 10 µg/mL.  Discard whatever is not used on the day of use.  6)      Grid plates � Purchase from Fisher Scientific (#08-757-149) 7)      Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain

Southern Hybridization Protocols

Primers Solution 7 µ l distilled water Mix briefly. Pipette up and down a tip of alpha - 32P with the above mixed solution until the reddish color on pipette tip disappears. Add 1 µ l Klenow fragment, centrifuge briefly