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英文名称:X500 0,5ML TUBE & CAP RED
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文献和实验, a pair of frogs were mated (see Grainger Lab X. tropicalis Mating Protocol for details) and the resultant tadpoles were used in the following karyotyping protocol. 2. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Materials Bunsen burner Wire loop Petri plate or broth tube Bacterial culture Microscope slides Procedure 1. Pick up the inoculating loop and hold it pointed down into an open flame until the loop glows red
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