5ml Self Standing Screw-Cap Tu

Cell Cultures

from the cultures. Place the sample in a separate test tube (it need not be sterile). 2. Dilute 4 parts of stock Trypan Blue with 1 part of 5X saline and add 0.1 ml of the diluted dye to your sample. Mix gently. 3. Set up a hemocytometer and cover slip

线虫蛋白提取--Making Protein Gel Samples from Worms

for other strains to catch up. 6A. If you don't need embryos in your sample, can just add 1 ml gel sample buffer and boil the tube (use a screw cap eppendorf) for 5 minutes, spin the tube 10 min. to pellet debris, and transfer the supernatant to a new tube

DNA isolation from yeast

  DNA isolation from yeast 1) Start a 5 ml o/n culture 2) Pellet o/n culture in a purple screw cap tube (this may take several spins), and dump s/n. 3) Add 100 µl of STET to pellet and vortex briefly. 4) Add about a cap