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货号：T_70103-313-2E
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英文名称：BOTTLE SMPLE NM 500ML 48/CS
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文献和实验7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
Construction of BAC Libraries：Megabase DNA Isolation
Blender). b. Homogenize the tissue in 200 ml ice cold 1 x HB plus b-mercaptoethanol in the kitchen blender at speed 4 or "puree" for 10 to 30 seconds. c. Filter the homogenate into an ice cold 250 ml centrifuge bottle
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