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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_701MP113002011
中文名称:
英文名称:LB MEDIUM(CAPSULES)227G(0.5LB)
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
and T4 polynucleotide kinase (70031) from United States Biochemicals. 2. Incubate at 37degC for 30-60 minutes. 3. Heat the reaction at 65degC for 10 minutes to inactivate the kinase. G. Bacterial cell maintenance
Basic procedures for bacteria culture
a culture of the bacterial cell strain onto an agar plate of the respective medium, listed below, and incubate at 37degC overnight. E. coli strain Agar Medium/Liquid Media XL1BMRF' (Stratagene) LB-Tet JM101 M9 ED8767
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