- ￥1000 - 20000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_701MP113002016
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701MP113002016
中文名称：
英文名称：LB-MEDIUM(LRG CAPS)227G(0.5LB)
货期：热销品现货，其他请咨询
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
Large-scale yeast transformation大规模转化酵母【Yale University】
PEG off top of box. Put caps on. Resuspend by vortexing. To plate on solid medium: We plate 24 transformations (2 rows of deepwell) per Nunc omniplate, using the 12 channel pipettor to put 5ul of cell suspension (in residual PEG) onto the plate
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