赛默飞世尔Thermo Fisher 1.5ML VIAL

V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法

placemultiples of 5 × 105 cells in each vial. 7. To recover frozen cells rapidly thaw a cryovial, add 4 mL of warm growthmedium dropwise and centrifuge for 5 min at 1000g. 8. Seed the cells on 6-cm bacterial culture plates (Falcon; see Note 1) in 5 mL

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with deionized water, and mix Mouse osteocalcin standard, one vial, 200 ng, lyophilized: reconstitute standard with exactly 2.0 ml of sample buffer (yields a 100 ng/ml solution); use

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Triethylammonium acetate (TEAA) buffer: 0.1 M TEAA, pH 7 Reaction vial, 1.5 mL, also known as “Eppendorf cups,” made of polypropylene (Neolab, http://www.neolab.info