赛默飞世尔Thermo Fisher Kapillarsäu

Single Primer ("Semi-Random") P

in the second cycling regime. If that doesn't work, try changing primers. If problems persist, switch to the long method. Do the following PCR reaction: 15 µL H2O 3 µL 10xM-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

A quick RNA mini-prep for Neurospora mycelial cultures

15. Spin 10-20 min in microfuge. Wash twice with 70 EtOH and dry pellet. Resuspend in 200 µl of sterile RNase-free gd H2O. Store at -70°C for up to three months. Spectophotometric quantification may be done at this point. 16. Load 1 µl