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货号：T_70311774725
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英文名称：X25 TUBE TEST COD 300 - 3500 MG/L
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Procedures for In Vitro Production of Bovine Embryos
(ml)1.02.00Stock 22: Ca chloride (ml)1.02.00.50Stock 23: Mg chlor (ml)1.101.00.25pH7.47.37.4Osmolarity (mOsm)295-305275-285290-300TALP (Tyrode’s Albumin Lactate Pyruvate) Media 1. To prepare media, mix the ingredients as described in Table
A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
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