赛默飞世尔Thermo Fisher 36X24 SHEET

Procedures for In Vitro Production of Bovine Embryos

hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes

SDS-PAGE

. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue

优化你的类器官培养水凝胶选择

浓度会是储备细胞悬液浓度的1/5。   试剂 不同最终凝胶体积下的试剂体积，µL 巯基反应性RGD可降解聚合物 18 36 72 CD细胞可降解交联剂 2 4 8 细胞悬液 5 10 20 总 25 50 100 表1. TRUE1（TrueGel3D™水凝胶CD细胞可降解交联剂和RGD肽）构建