赛默飞世尔Thermo Fisher FLASK VOL R

V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法

(do not count the smaller crescent-shaped red blood cells). 4. Seed the cells at 2 × 105 cells/cm2 on tissue culture plastic (Nunc). We routinelyseed 15 mL of cells suspended in growth medium in a 75-cm2 flask at a density of1 × 106 cells/mL. Incubate

重组DNA的分离、克隆与测序实验

1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989). 2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.  

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

Sample buffer, one 60‐ml bottle Wash buffer, phosphate‐saline concentrate, 5×, one 100 ml bottle: transfer contents to a graduated cylinder, bring volume up to 500 ml