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英文名称:FLASK VOL RED STR 25ML 6/PK
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文献和实验V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
(do not count the smaller crescent-shaped red blood cells). 4. Seed the cells at 2 × 105 cells/cm2 on tissue culture plastic (Nunc). We routinelyseed 15 mL of cells suspended in growth medium in a 75-cm2 flask at a density of1 × 106 cells/mL. Incubate
; see for selection] Deionized water (resistivity ≥18 MΩ and apparent pH 5.5) Argon 25‐ to 50‐ml glass round‐bottomed flask (cleaned by piranha or base etching; see protocol
25 ul total vol 150 ml 150 ml 150 ml Genetic technology grade (800669) or low gel temperature (800259) agarose from Schwarz/Mann Biotech. 2. Add 20X TAE and ethidium bromide (EtBr), swirl
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