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文献和实验Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
Georgescu, R.E., Kim, S.S., Yurieva, O., Kuriyan, J., Kong, X.P. and O'Donnell, M. 2008. Structure of a sliding clamp on DNA. Cell 132:43‐54. Hollis, T. 2007
V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
in a glass Coplin jar (or beaker) filled with 100% acetoneand sonicated for 10 min using a water bath sonicator (Decon FS Minor, UltrasonicLtd). After sonication the chamber is rinsed six times with distilled water. Once rinsed place the chamber in a glass
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