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- Thermo Fisher
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- T_701MP116030050
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701MP116030050
中文名称：
英文名称：FASTRNA SPIN KIT 50 PREPS
货期：热销品现货，其他请咨询
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文献和实验Ograve;alkali-lysis%26Oacute; to release DNA which is then separated from the rest of the cellular components. Traditional methods have included the use of organic solvent extractions (e.g. phenol extraction was used for mini-preps) or ultracentrifugation (e.g
Small scale DNA preps for Neurospora crassa.
to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex
Large Scale Plasmid Preps: Qiagen/Cesium Method
transfections of mammalian cells. PROCEDURE: Grow 250 - 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4ºC for 10 min. Purify the plasmid
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