赛默飞世尔Thermo Fisher FASTRNA SPI

Mini-preps

Ograve;alkali-lysis%26Oacute; to release DNA which is then separated from the rest of the cellular components. Traditional methods have included the use of organic solvent extractions (e.g. phenol extraction was used for mini-preps) or ultracentrifugation (e.g

Small scale DNA preps for Neurospora crassa.

to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex

Large Scale Plasmid Preps: Qiagen/Cesium Method

transfections of mammalian cells. PROCEDURE: Grow 250 - 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4ºC for 10 min. Purify the plasmid