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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_70313171143
中文名称:
英文名称:500 ML Viscosity silicone oil standards 1.000
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文献和实验7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
b) Add 4 volumes of room temperature 100% ETOH; gently mix c) Centrifuge at 12,000g at room temperature for 30 minutes. DO NOT USE A CENTRIFUGE THAT IS COLD. d) Remove supernatant. e) Add 3 ml 70% ETOH, gently swirl and centrifuge at 12,000g
Procedures for In Vitro Production of Bovine Embryos
in 25 ml (4-7 x 106 /ml). To do so, add 10 ml sperm suspension to 90 ml water to kill sperm. Load 10 ml of sample onto a hemacytometer. Count the number of sperm in 5 squares (Figure 18) and multiply sperm number by 500,000 to determine concentration
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