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货号：T_70114-223-084
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英文名称：AXYGEN AGAROSE MS ,50G,1 UNIT
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文献和实验Mapping Networks of Protein‐Mediated Physical Interactions Between Chromatin Elements
109 cfu/µg DNA; e.g., XL10‐Gold ultracompetent cells from Stratagene; also see unit 1.8 ) LB plates with antibiotic of choice (e.g., ampicillin or kanamycin) and Xgal and IPTG (unit
DpnI mediated site-directed Mutagenesis
the amplification to work efficiently with pfu take a bit of fiddling, amplification with Ex taq appears very robust for large plasmids. REFERENCES Fisher, C. L., and Pei, G. K. (1997). Modification of a PCR -based site-directed mutagenesis method
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
GSH‐agarose beads (e.g., glutathione‐Sepharose 4B; Amersham Biosciences) Wash buffers 1 or 2, and 3 (see reciperecipes
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