赛默飞世尔Thermo Fisher VIAL W/20-4

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

【转贴】immunofluorescence staining

. Store at 4C to prolong fluorescence.Reagents and Supplies:PBS & TBS-T buffers:PBS: Standard PBS for mammalian cellsTBS-T: Tris HCl 20 mM, pH 7.4 NaCl 137mM Tween-20 0.1%PEM Buffer:Stock Solution for 1 Liter:Final Concentration: 400 mM Potassium PIPES, pH