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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70110-067-042
中文名称：
英文名称：VF HEATING MANTLE 500 ML
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
in a hybridization, denature the labelled DNA by heating to 95-100 ℃ for 5 min, then chilled on ice.Add 50 m l probes to each hybridization tubeHybridize o/n at 65 ℃Filter washing: preparation of washing solutionW1 = 2SSC-0.5% SDS —> 150ml kept at RT W1 = 0.5SSC
BP 152-5 (MW 121.14) (890 mM) 110g Boric acid Fisher (electroph.gr) BP 168-500 (MW 61.83) (20 mM) 40 mL 0.5M EDTA pH 8.0 H2O to 2L, check pH w. strip (take out and drizzle ~500 ul on pH strip, ~pH 8.5, filter sterilize 0.2
Immunohistochemisty-Enzymatic Protocol
. Immunohistochem. Mol. Morphol. 8:236. Tissue Fixation and Mounting - Cryostat Sections Fix the tissue by vascular perfusion with 500 - 700 mL of Fixative. Perfuse the animal with 400 mL of Sucrose Solution. Dissect the tissue
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