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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70110-067-042
中文名称:
英文名称:VF HEATING MANTLE 500 ML
货期:热销品现货,其他请咨询




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文献和实验Southern Hybridization Protocols
in a hybridization, denature the labelled DNA by heating to 95-100 ℃ for 5 min, then chilled on ice.Add 50 m l probes to each hybridization tubeHybridize o/n at 65 ℃Filter washing: preparation of washing solutionW1 = 2SSC-0.5% SDS —> 150ml kept at RT W1 = 0.5SSC
BP 152-5 (MW 121.14) (890 mM) 110g Boric acid Fisher (electroph.gr) BP 168-500 (MW 61.83) (20 mM) 40 mL 0.5M EDTA pH 8.0 H2O to 2L, check pH w. strip (take out and drizzle ~500 ul on pH strip, ~pH 8.5, filter sterilize 0.2
Immunohistochemisty-Enzymatic Protocol
. Immunohistochem. Mol. Morphol. 8:236. Tissue Fixation and Mounting - Cryostat Sections Fix the tissue by vascular perfusion with 500 - 700 mL of Fixative. Perfuse the animal with 400 mL of Sucrose Solution. Dissect the tissue
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