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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310786522
中文名称:
英文名称:DC-Fertigplatten SILGUR-25 UV 254 Format: 20x20 cm (VE=25Stck.)
货期:热销品现货,其他请咨询




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文献和实验UV Crosslinking of Proteins to Nucleic Acids
°, 68°, 90°, and 100°C water baths UV transilluminator (305 nm, 7000 µW/cm2 ; Fotodyne)
FINGERPRINTING PROTOCOL (AGAROSE GEL)
. Pour into 20X25 cm UV transparent trays. Insert comb. Use 200 ml of molten agarose. After gel is solidified, remove comb and place in electrophoresis unit with enough 1X TAE buffer to cover the gel. Load 1.75 µl of the restriction enzyme digestion
DNA fingerprinting - agarose gel
. After digestion, a brief centrifugation will collect DNA at the bottom of wells. Seal plates with foil tape and store at 4℃ if necessary. Prepare 1% agarose gels in 1X TAE: Cool molten agarose to 46℃ in a water bath with occasional stirring. Pour into 20X25 cm UV
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