赛默飞世尔Thermo Fisher Comb 1.5mm

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially

小RNA的Northern BLOT

cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick)  Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel:  Add 678 μl

Random Amplified Polymorphic DNA (RAPD)

of about 5mm. Allow the gel to solidify for about 20 min at room temperature. To run, gently remove the comb, place the tray in electrophoresis chamber, and cover (just until wells are submerged) with electrophoresis buffer (the same buffer