赛默飞世尔Thermo Fisher Nunc EasYFl

Easy Way to Clone Genes From a Phage Library

on top. When putting filters on, you don't want significant air bubbles beneath them. • Write an identifier with pencil on each filter. Consider making duplicate filters for each plate to pre-verify that any signal in the end is real. • Carefully lay the filter

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

酵母转化的几种方法

700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum