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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
the herbicide solution on the plantlets. Efficient recovery was achieved using the following protocol : seeds (up to 1 g : approximately 50,000 seeds) are mixed with 4-5 volumes of fine sand and sown on 30 cm X 30 cm trays containing medium sand. The trays
onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA. The reactions terminated with the long termination mix typically are divided in half and loaded onto two 0.15 mm X 70 cm X 20 cm denaturing
. and resuspend in 1.5mL TE/LiAC. Add 100ul cells to the plasmid DNAs (1-5 ug) and single-stranded carrier DNA (20ug) in a microfuge tube. The carrier DNA is produced by dissolving salmon sperm DNA in TE, sonicating it to reduce its viscosity, extracting
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