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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311851723
中文名称:
英文名称:X4800 TIP BECKMANN 20µL NSTE
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
in 4% paraformaldehyde to give a working concentration of 10 µg/mL. Discard whatever is not used on the day of use. 6) Grid plates � Purchase from Fisher Scientific (#08-757-149) 7) Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain
Southern Hybridization Protocols
distilled water or TE in a microcentrifuge tube by heating for 5 min in a boiling water bath; then immediately cooled on ice. Perform the following adddtions on ice: 1 µ l 500 µ M dATP 1 µ l 500 µ M dGTP 1 µ l 500 µ M dTTP 20 µ l 2.5x Random
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