赛默飞世尔Thermo Fisher 4 X 50 ML A

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C

蛋白印迹(Western Blot)常用试剂

　 WB010 蛋白酶＋磷酸酶抑制剂混合物(20X) 1ml 380 　 WB011 流式用破膜剂 50T 1300 Invitrogen原装 WB012 淋巴细胞分离液（小鼠、大鼠、猪、兔等） 250ml