- ￥4500
- Sino Biological
- 北京
- 40592-V08H9
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2(2019-nCoV)Spike RBD-His(W436R)Recombinant Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein (40592-V08H9), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein (40592-V08H9) is 20-50 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(W436R)-His Recombiant Protein (YP_009724390.1) (Arg319-Phe541(W436R)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(W436R)-His Recombiant Protein consists of 234 amino acids and predicts a molecular mass of 26.51 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验. and Nusse, R. (2006) PurifiedWnt5a protein activates or inhibitsbeta-catenin-TCF signaling depending onreceptor context. PLoS Biol , 4 , e115. 12. Reya, T., Duncan, A. W., Ailles, L., Domen,J., Scherer, D. C., Willert, K., et al. (2003)A role for Wnt
处理软件，BioWorks 3.1 ( Thermo Electron) 。 4. 注释 ( 1 ) 建议以一系列代表性的样品在加或不加 DTT/IAA 情况下验证还原和烷基化反应的效率（或无效性)。检查已鉴定肽段的数量，角蛋白的污染，和是否存在羧胺甲基化半胱氨酸（+ 57 Da) 。 ( 2 ) SpeedVac 会导致颗粒和干燥蛋白的溶解性减小，降低肽段的回收率。30 μl 的提取物在室温下需要过夜蒸发或在 4°C 蒸发 1~2 天。 ( 3 ) 液体接头应紧固耐用，足以运行数百个样品
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