- ￥4500
- Sino Biological
- 北京
- 40592-V08H113
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)（产品说明）
蛋白名称：SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452Q, F490S) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant C.37) which emerged in the Peru.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.50 kDa. As a result of glycosylation, it migrates as an approximately 33.47 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452Q, F490S) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant C.37) which emerged in the Peru.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.50 kDa. As a result of glycosylation, it migrates as an approximately 33.47 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Li Y, et al. Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.iScience, PubMed ID: 35607524
2, Yu L, et al. Peptide Binder with High-Affinity for the SARS-CoV-2 Spike Receptor-Binding Domain.ACS applied materials & interfaces, PubMed ID: 35713340
3, Zhang DQ, et al. Peroxide derivatives as SARS-CoV-2 entry inhibitors.Virus research, PubMed ID: 38081457
2, Yu L, et al. Peptide Binder with High-Affinity for the SARS-CoV-2 Spike Receptor-Binding Domain.ACS applied materials & interfaces, PubMed ID: 35713340
3, Zhang DQ, et al. Peroxide derivatives as SARS-CoV-2 entry inhibitors.Virus research, PubMed ID: 38081457
相关实验
观察到小鼠出现炎症和不良反应。检测分析灭活疫苗的免疫原性显示，小鼠体内可被诱导产生针对SARS-CoV-2的S蛋白和RBD特异性的IgG，且针对RBD特异性的IgG占S蛋白抗体反应高达50%，提示RBD是新冠病毒灭活疫苗的主要免疫原。 2. 定点插入人源化hACE2小鼠模型中检院实验动物资源研究所主导的研究团队首次报道成功构建定点插入hACE2-KI/NIFDC小鼠模型。该人源化小鼠构建策略是将hACE2基因定点插入小鼠mAce2基因启动子之下，并将tdTomato基因插入到hACE2基因的下游
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Sino Biological 新冠Spike RBD重组蛋白 His标签 RBD S蛋白
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