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北京泽平
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货号：T_70312388149
中文名称：
英文名称：STAND RING 100MM WITH ROD 140MM
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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
) Embryonic culture dish Prepare by pouring 3 mL of agar substrate into a 35-mm Petri dish (Fisher Scientific, 0875711YZ) (Chapman et al. 2001 ). Embryonic culture insert Prepare by pouring 1 mL of agar substrate into a Millicell
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
used in this section: PBS-glucose solution, Betadine 1) For each litter, take the two sterile 100 mm petri dishes, place them in the hood and fill with ˜10 mL of ice-cold PBS-glucose. These should then be placed in an ice bucket filled
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
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