赛默飞世尔Thermo Fisher STAND RING

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

) Embryonic culture dish Prepare by pouring 3 mL of agar substrate into a 35-mm Petri dish (Fisher Scientific, 0875711YZ) (Chapman et al. 2001 ). Embryonic culture insert Prepare by pouring 1 mL of agar substrate into a Millicell

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1.    2)      In a third conical tube, prepare

In Situ HYBRIDIZATION TO TISSUE SECTIONS

may be too warm. Suspend a chunk of dry ice over the block (ring stand). Possible problem: Sections are deformed as they're sliced. Potential remedies: Slicing too fast, slow down the stroke. Or knife and/or block are too warm, cool with dry ice