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英文名称：X10 BOTTLE 30ML NM 28MM CAP HDPE (PACK OF 10)
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文献和实验Monoclonal Antibody Production Protocol
for 5 - 10 min. Aspirate the medium, resuspend pellet and wash again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free
Remove the cell suspension (without disturbing the settled cell debris) and transfer to a 50ml conical tube. Add an additional 30ml of IMDM and pellet the cells at 1200 rpm for 5 - 10 min. Aspirate the medium, resuspend pellet and wash
Water aspirator or in‐house vacuum line Tygon tubing (Fisher) 4‐mL screw‐cap glass vials (Wheaton
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