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- 库存：
999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70311907781
中文名称：
英文名称：25 ML KCL 3M SAT. AGCL ELECTROLYTE 25ML
货期：热销品现货，其他请咨询
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
water bath, occasionally shaking, to get SDS into solution. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin
. 2 ml 1 M KCl 1 ml 1 M Tris-HCl, pH 7.6 1 ml 1 M MgCl2 0.1 ml 1 M DTT ddH2 O to 10 ml RNase T1 : 100 U/ul in 50 mM Tris-HCl, pH 7.6 100 μl RNase T1 (Sigma R-8251) (100,000 U/0.2 ml) 25 μl 1 M Tris-HCl, pH 7.6 375 μl ddH2 O 500 μl 10
A quick RNA mini-prep for Neurospora mycelial cultures
. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent
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