赛默飞世尔Thermo Fisher X25 RED STO

DNeasy 96植物技术：使用搅拌磨MM 300从新鲜植物叶片中分离DNA

it into the empty clamp（重要的是：插入微型收集管架的两个适配微孔板必须固定到搅拌磨上，以提供平衡。如果希望处理96个或更少样品，要对搅拌磨MM 300进行平衡处理，方法是用另一没有样品和缓冲液但装有碳化钨珠子的收集微型管与两个适配微孔板装配，并用空夹子加入固定）。b. Shake for 1.5 min at 30 Hz （以30 Hz 摇震1.5 min ）。IMPORTANT: Prolonging the disruption time may result in shearing of DNA （重要

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

from Genebridges. Electroporation was performed at 1.35 kV, 25 μF, 200 Ω using an Eppendorf electroporator (2510). Bacteria were incubated in 1 ml LB media at 37°C, 1100 rpm for 1�2 h before plating on agar plates conditioned with the appropriate antibiotic(s

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

at RT with horizontal rocking.  0.5x MBS 10 ml 20 ml 30 ml 37% formaldehyde 270 µl 540 µl 810 µl (3)  To stop the reaction, rinse fixed