赛默飞世尔Thermo Fisher COMB 25 SAM

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

植物叶蛋白western blotting

6.8) 10% SDS 10% APS TEMED   1.3ml 7.25ml 1.3ml 104µl 52µl 11µl Insert comb, allow to polymerize (30 minutes); remove comb just before loading samples. III. Gel

TRICINE GELS Protocol (For Low MW proteins (

are very active in SDS sample buffer and can cause severe degradation. Once heated, sample could sit at RT for a short time until loading, or at -20°C for a long time. For a gel thickness of 0.75mm and 15 wells applied 10-25ug protein of a complex mixture