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文献和实验Expression of Recombinant Antibodies in Mammalian Cell Lines
, multidomain molecules such as full-length MAbs or complex recombinant antibody fragments, eukaryotic systems such as mammalian or yeast expression have been utilized. Currently, nine USFDA-licensed Mabs for in vivo human use have been expressed from mammalian
Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1 ,2 ). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N -acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used
Purification of Baculovirus-Expressed Human DNA Topoisomerase I
of human topo I (htopo I) in this manner becomes both costly and laborintensive if milligram quantities are needed for structural studies. Although active htopo I has been overexpressed in mammalian cells (3 ), yeast (4 ), and Escherichia coli
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