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- c1115-1gm
- 2025年07月10日
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1 gram
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文献和实验Affinity Purification Of Antibodies
1. Conjugation of carrier (BSA) to peptide(1) Dissolve pure BSA 20mg/ml in 10mM phosphate, pH 7.0.(2) Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide ester succinimidyl 4- (N-maleimidomethyl)cyclohexane-1-carboxylate] 25mg/ml
AFFINITY PURIFICATION OF ANTIBODIES
Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide ester succinimidyl 4- (N-maleimidomethyl)cyclohexane-1-carboxylate] @ 25
AFFINITY PURIFICATION OF ANTIBODIES
AFFINITY PURIFICATION OF ANTIBODIES (Fisher et al.,1988, Cell 54: 813-822) Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide
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