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文献和实验Development of techniques for primary culture of C. elegans embryonic neurons
were blocked for 30 min. with 10% BSA in PBS, followed by overnight incubation in primary antibody at 4'C, three room-temperature washes in PBS, and a 1 hr incubation in secondary antibody at 37'C. Following three washes in PBS, the cultures
Analysis of Heme and Hemoproteins
transformations (cytochrome P-450 and peroxidases), and most recently, as sensors in 2-component regulatory systems (guanylate cyclase, FixL, and CooA). The ability of hemoproteins to carry out extremely diverse reactions arises largely from the protein
THE PURINE NUCLEOTIDE CONTENT IN HUMAN LEUKEMIA CELL LINESAbstract: The HPLC method was used to determine the purine nucleotide (ATP, ADP, AMP, GTP, GDP, GMP, NAD+) contents and the values of the adenylate energy charge (AEC) and guanylate energy
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