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文献和实验the ON to a transporter peptide [29] and consequently the expression of the gene product of the targeted gene is decreased. Studies have shown that gene silencing by ON–CPP constructs is highly effective in vivo and in vitro [30] and several genes have been targeted using
Identification of Protein Interactions by Far Western Analysis
to express in cells. This method is performed totally in vitro, and the proteins of interest can be prepared in a variety of ways. A protocol is also provided for determining the effects of specific peptide residues or post?translational modifications
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
in the catalyticdomain exhibits enhanced expression in the brain (4) . GSK3 was first identified as the third kinase known to phosphorylateglycogen synthase (hence its name) as part of the insulinmediatedregulation of glucose metabolism (5) . Since then, it hasbeen
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