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文献和实验), whereas the internal and external RT-qPCRs were specifically designed to detect endogenous glyceraldehyde-3-phosphate dehydrogenase mRNA and a synthetic RNA, respectively. To maximize the sensitivity of the assay, the primer concentrations of the internal/external
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
and volumes for ppt.need 1.4ml ppt/100mm (half is 2x HeBS, half is DNA/CaCl2 mixture).20-30ug of total DNA required per 100mm plate.5-20ug reporter plasmid.3-4ug alpha-globin internal control.pUC19 to bring to 20-30ug total DNA.Aliquot volume of 2x HeBS
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
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